The FAD Project
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چکیده
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منابع مشابه
FAD oxidizes the ERO 1 - PDI electron transfer chain : The role of membrane integrity q , qq
The molecular steps of the electron transfer in the endoplasmic reticulum from the secreted proteins during their oxidation are relatively unknown. We present here that flavine adenine dinucleotide (FAD) is a powerful oxidizer of the oxidoreductase system, Ero1 and PDI, besides the proteins of rat liver microsomes and HepG2 hepatoma cells. Inhibition of FAD transport hindered the action of FAD....
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Many human diseases including cancer have been associated with altered cellular metabolism and a changed oxygen consumption in cells. Fluorophores are sensitive to their local environment due to their long life times in transient dark states. A recent study successfully utilized this sensitivity to image differences in oxygen concentrations in cells using transient state (TRAST) microscopy toge...
متن کاملBioelectrode based on reconstitution of a dye-linked L-proline dehydrogenase on gold electrode modified by PQQ and FAD
An electrochemical sensor was developed by reconstituting pyrroloquinoline quinone (PQQ), flavin adenine dinucleotide (FAD) and L-proline dehydrogenase on gold electrode. Reversible redox peaks and low redox potential were obtained from cyclic voltammograms, proved that the attached PQQ and FAD accelerated electron communication between enzyme active site and gold electrode surface. It is expec...
متن کاملFabrication of a polymer/mediator composite modified electrode and its application to electrochemical detection of iodate
A polymer/mediator composite modified glassy carbon electrode (GCE) is fabricated for electrochemical detection of iodate (IO3 ). Flavin adenine dinucleotide (FAD) is chosen as mediator for sensing iodate, and 3,4 ethylenedioxythiophene (EDOT) is chosen for entrapping FAD and forming a conducting polymeric matrix, poly(3,4 ethylenedioxythiophene) (PEDOT). The PEDOT/FAD modification layer is ele...
متن کاملIn Situ Ftir Thin-layer Reflectance Spectroscopy of Flavin Adenine Dinucleotide at a Mercury/gold Electrode
In this work, in situ FTIR thin-layer spectroelectrochemistry has been employed at a flat mercury-based electrode to obtain, uniquely, the spectrum of the reduced form of flavin adenine dinucleotide (FAD) in neutral (pH 7) solutions. The flat mercury surface was achieved by the open-circuit amalgamation of gold and it was found to display the advantageous electrochemical properties, including t...
متن کاملMolecular dynamics simulations of a DNA photolyase protein: High-mobility and conformational changes of the FAD molecule at low temperatures
A molecular dynamics (MD) simulation is performed on a DNA photolyase to study the conformational behavior of the photoactive cofactor flavin adenine dinucleotide (FAD) inside the enzyme pocket. A DNA photolyase is a highly efficient light-driven enzyme that repairs the UV-induced cyclobutane pyrimidine dimer in damaged DNA. In this work, the FAD conformational and dynamic changes were studied ...
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